The interaction between human blood-coagulation factor VIII and von Willebrand factor. Characterization of a high-affinity binding site on factor VIII.

نویسندگان

  • A Leyte
  • M P Verbeet
  • T Brodniewicz-Proba
  • J A Van Mourik
  • K Mertens
چکیده

The interaction between human Factor VIII and immobilized multimeric von Willebrand Factor (vWF) was characterized. Equilibrium binding studies indicated the presence of multiple classes of Factor VIII-binding sites on vWF. The high-affinity binding (Kd = 2.1 x 10(-10) M) was restricted to only 1-2% of the vWF subunits. Competition studies with monoclonal antibodies with known epitopes demonstrated that the Factor VIII sequence Lys1673-Arg1689 is involved in the high-affinity interaction with vWF.

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عنوان ژورنال:
  • The Biochemical journal

دوره 257 3  شماره 

صفحات  -

تاریخ انتشار 1989